A functional human Poly(A) site requires only a potent DSE and an A‐rich upstream sequence
Identifieur interne : 002751 ( Main/Exploration ); précédent : 002750; suivant : 002752A functional human Poly(A) site requires only a potent DSE and an A‐rich upstream sequence
Auteurs : Nuno Miguel Nunes [Royaume-Uni] ; Wencheng Li [États-Unis] ; Bin Tian [États-Unis] ; André Furger [Royaume-Uni]Source :
- The EMBO Journal [ 0261-4189 ] ; 2010-05-05.
English descriptors
- Teeft :
- Aauaaa, Additional plasmids, Adenosine, Alternative cleavage, Alternative sites, Anking, Anking regions, Anking sequences, Antisense riboprobe, Auuaaa, Auuaaa hexamer, Auxiliary sequence elements, Auxiliary sequences, Bioinformatics, Bioinformatics analysis, Biol, Biology organization, Biology organization sequences, Canonical, Canonical hexamer, Canonical hexamers, Canonical sites, Cell biol, Cells transfected, Cells transiently transfected, Cleavage, Cleavage site, Cleavage stimulation factor, Clone, Composite probe, Core sequences, Cpsf, Cstf, Deletion, Direct cleavage, Ds, Embo, Embo journal, Exact test, Gene, Hexamer, Hexamer mutations, Hexamers, Higher frequency, Human genes, Human sites, Intronless, Intronless genes, Junb, Junb site, Little effect, Many noncanonical sites, Mc4r, Mc4r site, Mrna, Mrna polyadenylation sites, Mutant plasmids, Mutation, Noncanonical, Noncanonical site, Noncanonical sites, Nucleic, Nucleic acids, Nucleotide, Nunes, Plasmid, Point mutations, Polyadenylation, Polyadenylation factor, Polymerase, Primer, Proc natl acad, Processing machinery, Processing signals, Processing site, Processing sites, Proudfoot, Readthrough, Receptor, Sequence element, Sequence elements, Site types, Supplementary figure, Terminal intron removal, Tian, Transcript, Transfected, Uguan, Uguan elements, Uguan motifs, Uguan sequences, Wilusz.
Abstract
We have analysed the sequences required for cleavage and polyadenylation in the intronless melanocortin 4 receptor (MC4R) pre‐mRNA. Unlike other intronless genes, 3′end processing of the MC4R primary transcript is independent of any auxiliary sequence elements and only requires the core poly(A) sequences. Mutation of the AUUAAA hexamer had little effect on MC4R 3′end processing but small changes in the short DSE severely reduced cleavage efficiency. The MC4R poly(A) site requires only the DSE and an A‐rich upstream sequence to direct efficient cleavage and polyadenylation. Our observation may be highly relevant for the understanding of how human noncanonical poly(A) sites are recognised. This is supported by a genome‐wide analysis of over 10 000 poly(A) sites where we show that many human noncanonical poly(A) signals contain A‐rich upstream sequences and tend to have a higher frequency of U and GU nucleotides in their DSE compared with canonical poly(A) signals. The importance of A‐rich elements for noncanonical poly(A) site recognition was confirmed by mutational analysis of the human JUNB gene, which contains an A‐rich noncanonical poly(A) signal.
Url:
DOI: 10.1038/emboj.2010.42
Affiliations:
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effect</term><term>Many noncanonical sites</term><term>Mc4r</term><term>Mc4r site</term><term>Mrna</term><term>Mrna polyadenylation sites</term><term>Mutant plasmids</term><term>Mutation</term><term>Noncanonical</term><term>Noncanonical site</term><term>Noncanonical sites</term><term>Nucleic</term><term>Nucleic acids</term><term>Nucleotide</term><term>Nunes</term><term>Plasmid</term><term>Point mutations</term><term>Polyadenylation</term><term>Polyadenylation factor</term><term>Polymerase</term><term>Primer</term><term>Proc natl acad</term><term>Processing machinery</term><term>Processing signals</term><term>Processing site</term><term>Processing sites</term><term>Proudfoot</term><term>Readthrough</term><term>Receptor</term><term>Sequence element</term><term>Sequence elements</term><term>Site types</term><term>Supplementary figure</term><term>Terminal intron removal</term><term>Tian</term><term>Transcript</term><term>Transfected</term><term>Uguan</term><term>Uguan elements</term><term>Uguan motifs</term><term>Uguan sequences</term><term>Wilusz</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">We have analysed the sequences required for cleavage and polyadenylation in the intronless melanocortin 4 receptor (MC4R) pre‐mRNA. Unlike other intronless genes, 3′end processing of the MC4R primary transcript is independent of any auxiliary sequence elements and only requires the core poly(A) sequences. Mutation of the AUUAAA hexamer had little effect on MC4R 3′end processing but small changes in the short DSE severely reduced cleavage efficiency. The MC4R poly(A) site requires only the DSE and an A‐rich upstream sequence to direct efficient cleavage and polyadenylation. Our observation may be highly relevant for the understanding of how human noncanonical poly(A) sites are recognised. This is supported by a genome‐wide analysis of over 10 000 poly(A) sites where we show that many human noncanonical poly(A) signals contain A‐rich upstream sequences and tend to have a higher frequency of U and GU nucleotides in their DSE compared with canonical poly(A) signals. The importance of A‐rich elements for noncanonical poly(A) site recognition was confirmed by mutational analysis of the human JUNB gene, which contains an A‐rich noncanonical poly(A) signal.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Oxfordshire</li></region><settlement><li>Oxford</li></settlement><orgName><li>Université d'Oxford</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Nunes, Nuno Miguel" sort="Nunes, Nuno Miguel" uniqKey="Nunes N" first="Nuno Miguel" last="Nunes">Nuno Miguel Nunes</name></region><name sortKey="Furger, Andre" sort="Furger, Andre" uniqKey="Furger A" first="André" last="Furger">André Furger</name><name sortKey="Furger, Andre" sort="Furger, Andre" uniqKey="Furger A" first="André" last="Furger">André Furger</name></country><country name="États-Unis"><noRegion><name sortKey="Li, Wencheng" sort="Li, Wencheng" uniqKey="Li W" first="Wencheng" last="Li">Wencheng Li</name></noRegion><name sortKey="Tian, Bin" sort="Tian, Bin" uniqKey="Tian B" first="Bin" last="Tian">Bin Tian</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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